Quercetin is extracted in ethylacetate fraction. LC–MS analysis of OsB and OsE showed the presence of luteolin, apigenin, rosmarinic, chlorogenic, caffeic acid and their derivatives. EC 50 values of different fractions for DPPH anion, ABTS cation free radical scavenging and phosphomolybdate reducing property were significantly and positively correlated with TPC and TFC. OsE also had significantly high TPC (202.71 ± 5.5 mg GAE/g extract).
OsB was rich in both total polyphenolic content (TPC) (212.26 ± 6.3 mg GAE/g extract) and total flavonoid contents (TFC) (54.51 ± 3.5 mg QE/g extract).
The leaves of Ocimum sanctum were extracted in methanol (OsM) and sequentially fractionated with n-hexane (OsH), ethylacetate (OsE) and butanol (OsB) to find the best extraction solvent for antioxidants from the herb known for its medicinal values.
0 kommentar(er)
